Approche d'une analyse quantitative de l'effect d'Apis mellifica sur la dégranulation des basophiles humains in vitro

Author(s): Aubin, M., Benveniste, J.

Journal/Book: J Innov Technol Biol Med. 1986; 7: 64-68.

Abstract: In vitro human basophil degranulation test (HBDT) is routinely used for the diagnostic of allergic diseases. It explores the allergic sensitivity at the cellular level, probably through an IgE-dependent sensitization of the basophil. We have measured the effect of an homeopathic drug, Apis Mellifica, on the in vitro degranulation of basophils sensitized to common allergens. The percentage of degranulation induced by the allergen is enhanced by 27,4% after incubation with Apis Mellifica 5CH and is decreased by 58.3% and 60.8 % after incubation with Apis Mellifica 9CH and 15CH respectively. This effect may be due to the regulation of cellular phospholipase A2 activity by constituents of Apis Mellifica, namely mellitin, a phospholipase A2 activator, and phospholipase A2 itself.

Keyword(s): allergic diseases

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