The Relation of Fluoride to Other Inorganic Substances in vivo* (30471)

Journal/Book: Reprinted from PROCEEDINGS OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE 1965 v120 150-154. 1965;

Abstract: Introduced by R. M. Archibald The Rockefeller Institute New York City Copyright 1965 by the Society for Experimental Biology and Medicine *I am indebted to Dr. R. M. Archibald for encouragement during the course of this work. The technical assistance of Mr. Compton de Abreu and Miss Lucia Seccone was invaluable. This study was supported in Part by U.S.P.H.S. Grant AM-03347. Â† Present address: Kanematsu Memorial Inst. Sydney Hospital Sydney Australia. The recognition of toxicity produced by high concentrations of fluoride in humans was reported as early as 1899 (1) . In 1932 Møller and Gudjonsson (2) first described pathological changes in the bones in workers engaged in crushing and refining cryolite and ascribed the changes to ingestion of fluoride. The curious sclerosis which appeared in the bones of the affected subjects did not cause any trouble unless the ligaments and the muscular attachments had become involved. MøIler and Gudjonsson suggested the use of cryolite or of "some compound of fluorine such as sodium fluoride" to treat bone diseases that produced pronounced osteoporosis. They quote the work of Brissemoret (3) in which similar therapy for bone diseases had been proposed. Rich and Ensinck (4) reported results of observations in patients with osteoporosis or with Pagets' disease who had been treated with sodium fluoride over a period of 14 weeks. The work described here in which rats were used as experimental animals shows that ingestion of fluoride can produce alterations in the absorption and excretion of minerals. Differences in mineral content of bone and of teeth when a high concentration of fluoride is given have also been demonstrated. Materials and methods. Thirty-one female rats (Charles River Laboratories) of mean weight 40 g were placed an a synthetic diet (5) with fluoride omitted from the trace mixture used. Analysis of the diet for fluorine showed the concentration to be 1.02 p.p.m. The diet was prepared and tube fed as previously described (6). Each animal initially received 4 g of the diet per day the amount being gradually increased until the fifth day when 8 g were fed. The food intake thereafter was maintained at 8 g. ... ___MH

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