Binding patterns of mistletoe lectins I, II and III to microglia and Alzheimer plaque glycoproteins in human brains

Journal/Book: Acta histochem. 96 (4), 399-403. 1994;

Abstract: Glycoconjugates of microglial cells and in some cases thoseglycoconjugates present in the amyloid plaques in Alzheimer's disease inthe cerebral cortex can be stained with a lectin from mistletoe (ML-I)using a labour-intensive and time-consuming indirect immunoperoxidasetechnique. In order to simplify the staining method and to test thestaining characteristics of the other recently isolated mistletoelectins (ML-II, ML-III), biotinylated MLs I-III were used together withan avidin-alkaline phosphatase-complex for visualisation. Our findingsindicate that this new improved technique can also be used for detectionof microglial cells and is considerably faster than the old method. Inaddition to microglial cells, ML-I labelled plaque glycoproteinspossibly indicating that glycoconjugates derived from microglia can bedetected in plaques. In contrast to ML-I, both ML-II and ML-III did notstain microglial cells.

Keyword(s): Medical:microglia

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