Efficient induction of superoxide release from human neutrophils by the galactoside

Journal/Book: Biol. Chem. Hoppe-Seyler 374 (4), 237-243. 1993;

Abstract: The immunomodulatory galactoside-specific lectin from Viscum album (VAA)induces superoxide anion (O2.-) release from human neutrophils. Amongtwelve tested lectins, VAA, has the highest activity, clearly surpassingthe effect of the human beta-galactoside-specific lectin (galaptin). Itsreactivity is blocked in the presence of lactose and is strictlydependent on the carbohydrate-binding B-subunit. The toxic A-subunit ofthe lectin does not elicit a response. The VAA-induced respiratory burstis less sensitive to addition of adenosine and theophylline than theconcanavalin A-mediated reaction. Other modulators like amiloride (up to100 microM), trifluoperazine and N-ethylmaleimide reveal less pronounceddifferences, and indomethacin, colchicine (up to 100 microM) as well ascytochalasin B act as stimulators of lectin-induced O2.- release fromneutrophils. The O2.- production in the presence of small concentrationsof VAA (0.1-20 micrograms/ml) is increased by addition ofN-formylmethionyl-leucyl-phenylalanine (FMLP), phorbol 12-myristate13-acetate (PMA) or digitonin, respectively, whereas concanavalin A (ConA) or wheat germ agglutinin (WGA) fail to affect the VAA-dependentextent of the respiratory burst. These results substantiate that theVAA-induced O2.- release from human neutrophils can be enhanced by otherclasses of inductors. Author.

Keyword(s): ADENOSINE/PD (pharmacology)

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Efficient induction of superoxide release from human neutrophils by the galactoside - specific lectin from VISCUM ALBUM