Effects of dilutions of pollens, histamine and arsenic on in vitro and ex vivo histamino-release of allergic subjects

Author(s): Santais, M. -C., Grossriether, H. , Callens, E. , Ruff, F.

Journal/Book: Proc GIRI. 1988; 2: 14.

Abstract: Several authors using the human basophil degranulation test (TDBH) have reported the effects of very low doses of histamine, PAF-acether or Apis Mellifica. In vitro or ex vivo histamino-release (HR) on total blood may be proposed as an alternative test to explore immediate hypersensitivity. In a previous study, we experimented Histaminum 7CH (10-14), Pollens 9CH (10-18) et Arsenicum album 15CH (10-30) on two groups of 6 grass pollens or D. pteronyssinus lergic patients. These substances were ten-fold diluted in Tyrode's solution just prior to assay and then coded. The released histamine by the basophils was measured by automated fluorometry in the supernatant and the pellet after

Keyword(s): hom. br. exp. imm. hum. allergy. histamine. arsenicum. pollen

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