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October 2022

Automated Sequential Sample Analysis for Some Amino Acids by Elution Chromatography

Journal/Book: Reprinted from ANALYTICAL CHEMISTRY Volume 40 Number 6 Pages 919-923 1968. 1968;

Abstract: Department of Health and Safety United Kinndom Atomic Energy Authority Chapelcross Dumfriesshire Scotland A new technique for automatically loading a chromatographic column is illustrated by its application to a Single-buffer-elution sequential analysis for a number of amino acids. When using a Technicon AutoAnalyzer to monitor the column eluate peak resolution and symmetry are comparable to manual loading and the relative standard deviation on an overnight run of seven consecutive analyses is 3%. The technique is based an two chromatographic Pumps of differing characteristics one loading the sample the other eluting it and regenerating the column. One of the main features of the method is that the additional chromatographic equipment including sample loading apparatus elution valves and programmer can be easily and inexpensively constructed. Sample cooling facilities are provided. The underlying principles limitations and possible further applications are briefly discussed. . . .


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