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October 2022

A Method for the Determination of Extracellular Space with 3H Inulin

Journal/Book: Reprinted from CLINICAL CHEMISTRY Vol. 13 No. 11 November 1967. 1967;

Abstract: Copyright (c) by American Association of Clinical Chemists HOEBER MEDICAL DIVISION of Harper & Row Publishers Printed in U.S.A. From the Departments of Pediatrics and of Physiological Chemistry The Ohio State University College of Medicine and The Children's Hospital Research Foundation Columbus Ohio 43205. Supported by Research Grants NB-05114 and FR-58 from the U. S. Public Health Service and by General Research Support Grant FR-05409 from the College of Medicine The Ohio State University. Presented in part at the Annual Meetings of the Midwest Society for Pediatric Research Chicago Ill. November 1965. The authors wish to thank Dr. Samuel Meites for helpful discussions. Received for publication Feb. 27 1967; accepted for publication Apr. 29 1967. A method for the extraction and quantitation of 3H inulin from tissues is described. This method does not require either combustion technics or time-consuming acid extraction procedures. Inulin is as soluble in the liquid scintillation medium as it is in water. Therefore hot-water tissue extracts without further treatment were employed to quantitate 3H inulin. Nearly 100% recovery was obtained when different 3H inulin internal standards were added either to water or to tissue homogenstes. The values obtained with this method for the extracellular space of different tissues of the rat agree with those reported in the literature. ___MH

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