Studies in Histochemistry LXIX. QUANTITATIVE HISTOLOGICAL DISTRIBUTION OF TOTALLY ACTIVATED PHOSPHORYLASE IN THE RAT ADRENAL AND THE INFLUENCE OF ADRENO-CORTICOTROPIC HORMONE IN VIVO*

Journal/Book: THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 237 No. 11 November 1962. 1962;

Abstract: From the Division of Histochemistry Department of Pathology Stanford University Medical School Palo Alto California Received for publication March 19 1962 * This investigation was supported by Research Grants H 06716 and RG 9227 from the National Institutes of Health United States Public Health Service. 1 The abbreviation used is: ACTH adrenocorticotropic hormone. SUMMARY. 1. The quantitative histological distribution of fully activated phosphorylase activity was. measured in adrenals of male albino rats with and without. subcutaneous injections of adrenocorticotropic hormone (25 mg per kg) 3 hours before death. Maximal activities were observed in the capsular and medullary zones but the hormonal treatment in vivo elicited increases inactivity in all zones-that cannot . be explained by conversion of the inactive to the active form of the enzyme. 2. A method for measurement of phosphorylase activity in microgram samples of tissue (microtome section) and in other small samples was developed based on acid hydrolysis of glucose 1-phosphate and colorimetric analysis of the glucose by using glucose oxidase. 3. The data were interpreted to lend additional support to the hypothesis that physiologically adrenocorticotropic hormone stimulates production of reduced triphosphopyridine nucleotide which is required for enzymatic hydroxylations in steroid hormone synthesis through a series of reactions starting with glucose 1-phosphate derived from glycogen by phosphorylase action. ___MH

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