A New Universal Buffer System

Journal/Book: (Reprinted from Nature Vol. 191 No. 4793 pp. 1099-1100 September 9 1961). 1961;

Abstract: Department of Biochemistry University of Leeds. DURING investigations of the effect of pH on the activity of certain enzymes it was found necessary to use a single buffer solution for measurements at widely different pH values. For various reasons the universal buffer solutions of Prideaux and Ward1 and Britton and Robinson2 were unsuitable and so the following solution was devised the pH value of which was found to vary almost linearly with added acid or alkali. . . . .

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