LEUKOKINETIC STUDIES. III. THE DISTRIBUTION OF GRANULOCYTES IN THE BLOOD OF NORMAL SUBJECTS*

Journal/Book: Reprinted from THE JOURNAL OF CLINICAL INVESTIGATION Vol. 40 No. 1 pp. 159-164 January 1961 Printed in U. S. A.. 1961;

Abstract: From the Department of Medicine University of Utah College of Medicine Salt Lake City Utah Submitted for publication August 15 1960; accepted September 14 1960 *This investigation was supported in part by research grants (C-2231 and CY 2349) from the National Cancer Institute and in part by a graduate training grant (2A-5098) from the National Institute of Arthritis and Metabolic Diseases Bethesda Md. Â† Fellow of the Leukemia Society Inc. New York N. Y. SUMMARY 1. When granulocytes are labeled in vitro with radioactive diisopropylfluorophosphate and returned to the circulation of the donor only about 50 per cent of the labeled granulocytes is present in the circulation at the completion of the infusion. It was postulated previously that the initial rapid egress of 50 per cent of the cells represented distribution of the injected granulocytes in a larger pool than that calculated from the blood volume and the venous granulocyte count. The experiments reported herein were designed to test the validity of this hypothesis. The following observations were made. a) An average of 78.8 per cent of the labeled granulocytes which were infused could be accounted for in the circulating blood when epinephrine was given with the infused cells. b) An average of 79 per cent of the labeled granulocytes was found in the circulation when the subjects exercised immediately following the completion of the Infusion. c) Neither epinephrine nor exercise increased the size of the total distribution compartment (total blond granulocyte pool) although both the drug and physical exertion increased the size of the circulating granulocyte pool. d) Granulocytes damaged by storage could not be prevented from leaving the circulation by the administration of epinephrine. e) When granulocytes were mobilized into the circulation 3 hours after the infusion of labeled granulocytes there was no change in the specific activity of the cells in the circulation. 2. These studies are interpreted as indicating that infused granulocytes are distributed in a total blood granulocyte pool (TBGP) made up of two compartments which are in rapid equilibrium with each other. These pools have been designated the circulating granulocyte pool (CGP) and the marginal granulocyte pool (MGP) . 3. The size of these pools has been measured in 45 normal male subjects. The mean values ± 1 SD expressed as number of cells X 107 per kg of body weight were as follows : TBGP 65.3 ± 22.7 ; CGP 30.7 ± 11.8; and MGP 34.6 - 15.6. ___MH

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