DETERMINATION OF HISTAMINE IN BLOOD AND URINE BY ABSORPTION ON AMBERLITE IRC

Journal/Book: Reprint from The Scandinavian Journal of Clinical & Laboratory Investigation. 1958. Vol. 10 No. 3.. 1958;

Abstract: From the King Gustaf V Research Institute and the Department of Clinical Physiology Karolinska sjukhuset Stockholm Sweden WITH SPECIAL REFERENCE TO THE NORMAL VALUES FOR HISTAMINE IN HUMAN BLOOD AND AN ANALYSIS OF THE SPECIFICITY OF THE METHOD SUMMARY 1. On the basis of a previously described method for extraction of histamine from urine a modified technique for determination of histamine in blood is described. The content of histamine in serum ranged from 0.6-2.8 µg per 100 ml of blood (mean 1.29 ± 0.17). The corresponding values for blood cells were 1.0-4.6 with a mean of 2.66 ± 0.33 µg per 100 ml of blood. 2. The specificity of the method was studied by passing mixtures of histamine and other smooth muscle-stimulating agents such as acetylcholine 5-hydroxytryptamine (5HT) substance P and potassium through the Amberlite column. At the subsequent biological assay of the eluates 5HT and potassium were found in the same fractions as histamine. 3. Because of this finding the earlier described method for extraction and determination af histamine (Dunér & Pernow 1956) was modified by a treatment of the eluates with alcohol-acetone in order to precipitate the salts and by addition of dihydroergotamine to the bath-fluid in order to make the intestine insensitive to 5HT. 4. Administration of an antihistaminic promethazine renders the biological assay of histamine impossible because of excretion of promethazine in the urine. However the promethazine can be eliminated by extraction with ether (Rhone-Poulenc 1954). The excretion of histamine in the urine was found not to be influenced by oral administration of promethazine.

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