Acta Crystallogr D Biol Crystallogr. 2003 Sep; 59(Pt 9): 1533-8.

The influence of an internal electric field upon protein crystallization using the gel-acupuncture method.

Mirkin N, Frontana-Uribe BA, Rodríguez-Romero A, Hernández-Santoyo A, Moreno A.

Instituto de Química, UNAM Circuito Exterior, C. U. Mexico, D. F. 04510, Mexico.

In this work, the influence of an internal electric field upon the crystallization of lysozyme and thaumatin is explored using a modified design of the gel-acupuncture setup. From a crystallographic point of view, the orientation of crystals that grow preferentially over different types of electrodes inside capillary tubes is also evaluated. Finally, the crystal quality and the three-dimensional structure of these proteins grown with and without the electric field influence are analyzed by means of X-ray diffraction methods.

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