Phytomedicine. 2003 ; 10(5): 422-6.

Trypanocidal activity of extracts from Brazilian Atlantic Rain Forest plant species.

Pizzolatti MG, Koga AH, Grisard EC, Steindel M.

Department de QuÃmica, LaboratÃ³rio de QuÃmica de Produtos Naturais, FlorianÃ³polis, SC, Brazil. [email protected]

The trypanocidal activity of crude hydro alcoholic extracts and several fractions of 13 plants from Brazilian Atlantic Rain Forest were tested in vitro against epimastigote and trypomastigote forms of Trypanosoma cruzi, the etiological agent of Chagas disease. Crude ethanol extracts with promising in vitro activity (DL50 between 5-10 microg/ml) against epimastigotes were fractionated by solvent partition and further tested against bloodstream form of the parasite. Activity against bloodstream parasites was observed in both dichloromethane and hexane fractions of Polygala sabulosa and P. paniculata.