Zhongguo Zhong Xi Yi Jie He Za Zhi. 2003 Apr; 23(4): 281-3.

[Effect of congsheng capsule on intracellular calcium concentration in mice after ischemic cerebral injury]

Zhao L, Xu QP, Li L.

Department of Pharmacology, Xuanwu Hospital, Capital University of Medical Sciences, Beijing Key Laboratory for Brain Aging, Beijing 100053.

OBJECTIVE: To investigate the effect of Congsheng Capsule (CSC) in intracellular Ca2+ concentration in cerebral intra-synaptosomes of ischemia/reperfusion mice and analogous ischemic injured neurons to estimate the mechanism of CSC in antagonizing ischemic cerebral injury. METHODS: The concentration of Ca2+ in cerebral intra-synaptosomes of mice and in neurocyte suspension of fetal mice were determined using Fura-2/AM double wavelength fluorescence spectrophotometer. RESULTS: CSC 3 g/kg and 9 g/kg through gastrogavage could relieve the degree of calcium overloading of intra-synaptosomes in mice after cerebral ischemia/reperfusion. The CSC containing serum could inhibit the elevation of Ca2+ content in neurocytes induced by 1 hour hypoxia and glutamate of high concentration (200 mumol/L). CONCLUSION: CSC plays a role in antagonizing ischemic injury through relieving the calcium overloading after cerebral ischemia.