Screening of 25 compounds isolated from Phyllanthus species for anti-human hepatitis B virus in vitro.

Huang RL, Huang YL, Ou JC, Chen CC, Hsu FL, Chang C.

National Research Institute of Chinese Medicine, No. 155-1, Sec. 2, Li Nung St., Peitou, Taipei, Taiwan, Republic of China.

Using an HBV-producing cell line and inhibition of the expression of the HBsAg and HBeAg as antiviral indicators, a study was conducted on 25 compounds isolated from four Phyllanthus (Euphorbiaceae) plants, including P. amarus Schum. & Thonn., P. multi florus Willd., P. tenellus Roxb. and P. virgatus Forst. f. It was found that niranthin (1), nirtetralin (3), hinokinin (5) and geraniin (13) at the non-cytotoxic concentration of 50 micro m, suppressed effectively both HBsAg and HBeAg expression, with the highest inhibition at 74.3%, 45.3%; 69.6%, 33.9%; 68.1%, 52.3%; 32.1%, 46.6%, respectively. Of these, niranthin (1) showed the best anti-HBsAg activity, while the most potent anti-HBeAg activity was observed with hinokinin (5).Copyright 2003 John Wiley & Sons, Ltd.

0951-418X 17 5 2003 May Screening of 25 compounds isolated from Phyllanthus species for anti-human hepatitis B virus in vitro. 449-53 Using an HBV-producing cell line and inhibition of the expression of the HBsAg and HBeAg as antiviral indicators, a study was conducted on 25 compounds isolated from four Phyllanthus (Euphorbiaceae) plants, including P. amarus Schum. & Thonn., P. multi florus Willd., P. tenellus Roxb. and P. virgatus Forst. f. It was found that niranthin (1), nirtetralin (3), hinokinin (5) and geraniin (13) at the non-cytotoxic concentration of 50 micro m, suppressed effectively both HBsAg and HBeAg expression, with the highest inhibition at 74.3%, 45.3%; 69.6%, 33.9%; 68.1%, 52.3%; 32.1%, 46.6%, respectively. Of these, niranthin (1) showed the best anti-HBsAg activity, while the most potent anti-HBeAg activity was observed with hinokinin (5). Copyright 2003 John Wiley & Sons, Ltd. National Research Institute of Chinese Medicine, No. 155-1, Sec. 2, Li Nung St., Peitou, Taipei, Taiwan, Republic of China. Huang Ray-Ling RL Huang Yu-Ling YL Ou Jun-Chih JC Chen Chien-Chih CC Hsu Feng-Lin FL Chang Chungming C eng Journal Article