J Environ Pathol Toxicol Oncol. 2003 ; 22(1): 59-67.

Studies on correlation of antimutagenic and antiproliferative activities of Juglans regia L.

Kaur K, Michael H, Arora S, H�rkÃ¶nen PL, Kumar S.

Department of Botanical Sciences, Guru Nanak Dev University, Amritsar, India. [email protected]

We investigated the effect of water and acetone extract of Juglans regia L. to evaluate its antimutagenic and antiproliferative activities. The antimutagenic study using TA98 and TA100 tester strains of Salmonella revealed the water and acetone extracts to be more effective than the benzene and chloroform extracts in inhibiting the revertants induced by 2-aminoflourene (2AF) in TA100 tester strains. The most effective extracts in the Ames assay were further evaluated using the Lucifer luciferase assay and in time course studies for antiproliferative activities using the Hoechst staining to observe apoptotic cell deaths. The acetone extract showed a correlation of antimutagenic activities in the Ames assay with its antiproliferative effect in different cell lines, while the water extract exerted its effect distinctly in each cell line. Further studies are still needed to evaluate the cytotoxicity in experiments carried out in vivo.