Zhongguo Zhong Yao Za Zhi. 2002 May; 27(5): 363-5.

[The changes of some chemical components in banxiaxiexintang decoction of different combinations]

Duan TX, Ma CH, Chen T, Lun LH, Okuma R, Li Y.

College of Chinese Pharmacy, Beijing University of Traditional Chinese Medicine, Beijing 100029, China.

OBJECTIVE: The contents of bererine, palmatine and glycyrrhizin acid in Banxiaxiexintang decoction of different combinations were determined by PR-HPLC. METHOD: A Shim-pack CLC-ODS column was used with a mobile phase of CH3CN-H2O (31:69; 0.005 moL.L-1 -pentanesulfonic acid sodium salt, H3PO4: pH 3.0) for bererine andpalmatine, which were detected at the wavelength of 275 nm. A YWG-C18 column was used with a mobile phase of CH3OH-H2O-HAc(62:37:1) for glycyrrhizin acid which was detected at the wavelength of 260 nm. RESULT: Each herbs' combination influences the contents of the 3 components. CONCLUSION: The experiment is an attempt to study the comical foundation of traditional Chinese prescription.