Zhongguo Zhong Xi Yi Jie He Za Zhi. 2002 Aug; 22(8): 610-2.

[Experimental study on effect of sini decoction on myocardial endothelin in myocardial ischemic rats]

Wu WK, Zhou L, Sun HL.

Institute of Integrative Traditional Chinese and Western Medicine, Sun Yat-Sen University, Guangzhou 510080.

OBJECTIVE: To study the molecular mechanism of effect of Sini Decoction (SND) on myocardial endothelin (MET) in myocardial ischemic rats. METHODS: Sprague-Dawley rats were randomly divided into 3 groups, the normal control group, the ischemia group and the SND group. Myocardial ischemia was produced by pituitrin in the latter two groups. The content, immunohistochemical assay and gene expression of MET-1 were determined in all the three groups and compared. RESULTS: The content of MET in the SND group was significantly lower than that in the ischemia group (P < 0.01). Immunohistochemical examination showed that MET-1 was mainly located at the cardiac muscle cells and vascular endothelial cells with the grey scale obviously lower in the ischemia group than that in the control group and the SND group (P < 0.01). While RT-PCR showed that the grey scale of PCR product band in the ischemia group was significantly higher than that in the other two groups (P < 0.01). CONCLUSION: SND could significantly lower MET content, it may be related to the effect of SND in inhibiting MET-1 gene expression and protein synthesis.