J Asian Nat Prod Res. 2002 Sep; 4(3): 171-4.

Callus cultures of Annona squamosa for the production of annonaceous acetogenins.

Wang LD, Qiu DY, Chen JY, Han YF, Zheng JH, Guo DA.

School of Pharmaceutical Sciences, Peking University, Beijing, China.

Callus cultures of Annona squamosa were induced using different explants including petals, seed contents (megagametophyte and embryo) and fruits (mesocarp). Growth of the calli induced from the explants was found to be influenced by the type, concentration and ratio of auxin vs. cytokinin. The content of squamocin (67.8 microg g(-1) dry weight) in calli cultured on Gamborg B-5 medium containing 5.0 mg l(-1) naphthalene acetic acid and 4.0 mg l(-1) zeatin was nearly seven times higher than that in intact fruits.