Cancer Lett. 2002 Sep; 183(1): 17-22.

Suppression of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine-induced DNA damage in rat colon after grapefruit juice intake.

Miyata M, Takano H, Takahashi K, Sasaki YF, Yamazoe Y.

Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba-ku, 980-8578, Sendai, Japan. [email protected]

The influence of grapefruit juice intake on 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP)-induced colon DNA damage was examined using comet assay in F344 rats given 60 mg/kg of PhIP by gavage. F344 rats allowed free access to grapefruit juice for 5 days experienced clearly reduced DNA damage in the colon to a 40% level of control rats. The suppression of PhIP-induced colon DNA damage depended on the grapefruit juice concentrations. The serum concentration of PhIP was compared between grapefruit juice-pretreated and non-pretreated rats, but showed no significant difference in the areas under their concentration-time curves, peak values and half lives of PhIP. Furthermore, no obvious difference was found in the liver capacity for mutagenic activation of PhIP in Ames assay between grapefruit juice-pretreated and non-pretreated rats. These results suggest that grapefruit juice suppresses PhIP-induced colon DNA damage by a mechanism independent of PhIP absorption in the intestine.