Fitoterapia. 2002 Apr; 73(2): 121-34.

OPLC and AMD, recent techniques of planar chromatography: their interest for separation and characterization of extractive and synthetic compounds.

Galand N, Pothier J, Dollet J, Viel C.

It is always important for the chemist to have good methods of separation, characterization and quantitative evaluation for one or several compounds resulting from a chemical reaction or an extraction procedure. In this domain the chromatographic techniques are choice methods, in particular Over Pressured Layer Chromatography (OPLC) and Automated Multiple Development (AMD). They are relatively recent methods whose use is unfortunately not yet generalized although they give very clean separation. In this paper we present numerous examples of the use of these two new types of planar chromatographies and especially the results we have obtained in the field of natural products on a wide variety of different structures: coumarins, flavonoids, anthocyanins, alkaloids and essential oils.