Zhongguo Zhong Xi Yi Jie He Za Zhi. 2001 Aug; 21(8): 611-4.

[Experimental study of compound salvia injection in preventing and treating chronic nephrotoxicity induced by cyclosporin A in rats]

Qiao BP, Tang XD, Ruan Q.

First Affiliated Hospital of Henan Medical University, Zhengzhou 450052.

OBJECTIVE: To explore the mechanism of protective effect of Compound Salvia Injection (CSI) on experimental cyclosporin A induced nephrotoxicity. METHODS: Rats were on low-salt diet and cyclosporin A (CsA) was administered once a day through gastrogavage at dosage of 30 mg/kg.d for 28 days. Expression of the mRNA for intrarenal transforming growth factor-beta 1 (TGF-beta 1) and renin was measured by reverse transcription-polymerase chain reaction (RT-PCR). Intrarenal expression of TGF-beta 1 and Collagen IV was determined by immunohistochemical assays. The effects of CSI on these changes were also evaluated. RESULTS: Chronic CsA-induced nephropathy might be correlated to TGF-beta 1 and renin mRNA up-regulation as well as matric proteins accumulation in interstitium. CSI could reduce these changes. CONCLUSION: Decreased CsA-related TGF-beta 1 and renin upregulation expression and accumulation of matrix proteins in the kidney might be related to the protective mechanism of CSI on CsA-induced chronic nephrotoxicity.