Zhongguo Zhong Xi Yi Jie He Za Zhi. 2001 Sep; 21(9): 680-3.

[Effect of dingxin recipe on intracellular free calcium, membrane potential and mitochondria membrane potential in normal and hypoxia myocytes of rats]

Jia YH, Sun XG, Zhao XS.

Faculty of TCM, First Military Medical University, Guangzhou 510515.

OBJECTIVE: To elucidate the molecular mechanism on anti-arrhythmic function of Dingxin Recipe (DXR). METHODS: Cardial myocytes isolated by trypsin method were cultured and labelled with various fluorescence stains. DXR contained serum induced changes of cytolic calcium concentration [Ca2+]i, cell membrane potential (MP) and mitochondria membrane potential (MMP) were determined with laser confocal microscopy. RESULTS: Hypoxia caused increase of [Ca2+]i and MMP, and decrease of MP, while DXR contained serum lowered the [Ca2+]i in normal or hypoxian myocytes, improved hypoxia induced MP reduction and maintain MMP at baseline level under hypoxia circumstances. CONCLUSION: Through inhibiting the hypoxia caused [Ca2+]i and MMP elevation and MP lowering, DXR displays its action in protecting myocardial cells to prevent and treat arrhythmia.