Phytother Res. 2001 Mar; 15(2): 127-30.

Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method.

Mensor LL, Menezes FS, LeitÃ£o GG, Reis AS, dos Santos TC, Coube CS, LeitÃ£o SG.

Departamento de Produtos Naturais e Alimentos, Faculdade de FarmÃ¡cia, Universidade Federal do Rio de Janeiro, Centro de CiÃªncias de SaÃºde, Bl. A, Ilha do FundÃ£o, Rio de Janeiro, 21941-590 RJ, Brazil.

Brazilian plant extracts belonging to 16 species of 5 different families (71 extracts) were tested against the stable DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) free-radical. The ability to scavenge DPPH radical was measured in these experiments by the discoloration of the solution. Ginkgo biloba and rutin, commonly used as antioxidants for medical purposes, were used as standards. Based on our results, we can say that as a general rule the ethanol extracts of plants belonging to the Verbenaceae family showed lower EC(50) values than the other plant extracts. Among the partitions, the more polar ones (ethyl acetate and n-butanol) are those that generally have higher antioxidant activity (AA).Copyright 2001 John Wiley & Sons, Ltd.