Zhongguo Zhong Xi Yi Jie He Za Zhi. 2000 Nov; 20(11): 824-7.

[Dynamic changes of serum pro-inflammatory cytokines and anti-inflammatory cytokines in patients with acute infection and the effect of Chinese herbal medicine intervention]

Qi W, Ren A, Zhang S.

Beijing Friendship Hospital, Beijing (100050).

OBJECTIVE: To study the dynamic changes of serum pro-inflammatory cytokines and anti-inflammatory cytokines in patients with acute infection and to explore the effect of 912 compound, a Chinese herbal preparation for intervention. METHODS: Forty patients with acute infection were randomly divided into 2 groups, 20 in the 912 group and 20 in the control group, and the levels of plasma endotoxin (lipopolysaccharide, LPS), serum pro-inflammatory cytokines, including tumor necrosis factor alpha (TNF alpha) and interleukin-6 (IL-6) and anti-inflammatory cytokines, including soluble TNF-RI, IL-4 and IL-10 were measured before and after treatment by using limulus reagent stroma azo-colored quantitative method and enzyme-linked immunosorbent assay (ELISA). RESULTS: The level of LPS increased at the early stage of acute infection, the levels of pro- and anti-inflammatory cytokines were all elevated in various degrees, particularly obvious in IL-6, sTNF-RI, IL-4 and IL-10 and the elevations of the two were basically parallel. The TNF alpha, IL-6 and sTNF-RI were positively related with APACHE III scoring. The levels of LPS and inflammatory cytokines reduced to a different extent in accord with the improvement of condition after treatment, the levels of LPS, TNF alpha, IL-6 and IL-10 lowered more significantly in the 912 group than those in the control group (P < 0.05). CONCLUSIONS: LPS, pro-inflammatory and anti-inflammatory cytokines play important roles in the acute infection process, they were important indexes for evaluating severity of infectious diseases. The Chinese herbal preparation 912 compound could reduce the blood levels of LPS, TNF alpha, IL-6, IL-10 and prevent the decrease of sTNF-RI so as to promote the recovery of patients.