J Ethnopharmacol. 1999 Sep; 66(3): 271-6.

Acute toxicity and general pharmacological effect on central nervous system of the crude rhizome extract of Kyllinga brevifolia Rottb.

HelliÃ¶n-Ibarrola MC, Ibarrola DA, Montalbetti Y, Villalba D, Heinichen O, Ferro EA.

Pharmacology Department, Faculty of Chemical Sciences, National University of Asuncion, Paraguay. [email protected]

Acute toxicity and general pharmacological activities of the crude hydro-alcoholic rhizome extract of Kyllinga brevifolia Rottb., a popular medicine used in Paraguay, were investigated on mice. The intraperitoneal LD50 was found to be 575 mg/kg. Oral administration of doses up to 3.0 g/kg did not provoke any toxic symptoms. Oral administration of 100 mg/kg of the extract induced a significant increase in gastrointestinal transit. In open field studies, a decrease of spontaneous locomotor activity, piloerection, passivity, palpebral ptosis, catatonia and a stereotyped behaviour was produced by the extract when administered orally (1, 10 and 100 mg/kg). A significant decrease in respiration rate was observed (1, 10 and 100 mg/kg, p.o.) using a continuous flow respiration system. Lastly, doses of 1, 10 and 100 mg/kg, p.o. of the extract produced a significant increase in the hypnotic effect induced by pentobarbital in a dose-dependent manner. The latest effects could probably explain its rational use in traditional medicine to alleviate stress or as a sedative agent.