Life Sci. 1998 ; 63(12): 995-1003.

Proliferative effects of oxidized low-density lipoprotein on vascular smooth muscle cells: role of dietary habits.

L�hteenm�ki TA, Korpela R, Tikkanen MJ, Karjala K, Laakso J, Solatunturi E, Vapaatalo H.

Institute of Biomedicine, Department of Pharmacology and Toxicology, University of Helsinki, Finland. [email protected]

The effects were studied of native, partially-oxidized and totally-oxidized human low-density lipoprotein (LDL) on the proliferation of cultured rat aortic smooth muscle cells (VSMC), measured as an altered DNA synthesis. The LDL was obtained from three different human long-term diet groups (a control diet rich in saturated fats, a vegetarian diet, and a fish diet). The oxidized LDLs were prepared by oxidizing the LDL with copper sulfate. The DNA synthesis was measured by [3H]-thymidine incorporation into the DNA. The partially-oxidized LDL was the most potent promoter of DNA synthesis compared to the native or totally-oxidized LDL of the same diet group. The partially-oxidized LDL had a true mitogenic effect in the absence of exogenous growth factors. The native and totally-oxidized LDL induced a significant increase in DNA synthesis, if they were obtained from the fish diet group. This study suggests an enhanced proliferative effect of partially-oxidized LDL on VSMC growth.