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Comparison of methods for determination of total antioxidant status: application to analysis of medicinal plant essential oils

Author(s): Mantle, D., Anderton, J. G. , Falkous, G. , Barnes, M. , Jones, P. , Perry, E. K.

Journal/Book: Comp Biochem Physiol. 1998; 121(4): 385-391.

Abstract: The free radical scavenging capacity of a wide range of plant oil extracts, principally those used in traditional European herbal medicine (with novel therapeutic potential for patients with degenerative disorders of the CNS), has been compared in vitro. The antioxidant capacity of individual plant extracts was determined via three complementary assay procedures, based on: (i) attenuation of the generation of ABTS(.+) radical (quantitated colorimetrically), by a metmyoglobin catalyst/hydrogen peroxide system; (ii) inhibition of iodophenol enhanced chemiluminescence by a horseradish peroxidase/perborate/luminol system; (iii) protection of a target enzyme (human brain alanyl aminopep rders.

Keyword(s): cam. diagn. antioxidant-status


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