Biol Trace Elem Res. 1996 Oct-Nov; 55(1-2): 173-9.

A high resolution PIXE measurement for blood plasma ultrafiltrate. Application to loosely bound copper.

Kupila-Rantala T, Dabek JT, HyvÃ¶nen-Dabek M.

Department of Physics, University of Helsinki, Finland.

A new technique for investigation of elemental concentrations in subfractions of blood plasma is presented. The method is composed of the ultrafiltration of plasma in the presence of ethylenediaminetetraacetic acid (EDTA) and the measurement of the elemental composition by proton-induced X-ray emission (PIXE). The blood samples were collected from both healthy persons and patients suffering from breast cancer. The main emphasis in this study was on the determination of loosely bound copper (Cu) in plasma subfractions containing substances with molecular mass under 10,000, but zinc (Zn) and iron (Fe) contents of these fractions were also determined. The detection limits obtained with this method for Cu, An and Fe were approximately 10 ppb (wet wt).