Kaohsiung J Med Sci. 1996 Jan; 12(1): 18-24.

The effect of "chi-han (hot nature)" Chinese herbs on the secretion of IL-1 beta and TNF-alpha by mononuclear cells.

Chang JY, Yang TY, Chang CP, Chang JG.

Department of Molecular Medicine and Chinese Medicine, Taipei Municipal Jen-Ai Hospital, Taiwan, Republic of China.

The effect of "chi-han (hot nature)" Chinese herbs on the secretion of the cytokines IL-1 beta and TNF-alpha was investigated by studying the in vitro effect of the "hot nature" Chinese herbs Radix aconiti, Evodia rutaecarpa, Zingiberis rhizoma, and Cortex cinnamomi. An ethanolic extract of each of these Chinese medicinal herbs was added to human peripheral mononuclear cell culture medium and allowed to react for various specified lengths of time. The culture medium supernatants were collected and tested for their cytokine levels at specified time intervals. We found different reaction patterns of cytokine secretion among these "hot nature" Chinese herbs. Radix aconiti extract showed an augmentative effect on the secretion of cytokines IL-1 beta and TNF-alpha with 20% to 50% concentrations of the pure herbal extract, especially when the reaction time was 18 or 24 hours. Evodia rutaecarpa extract showed a biphasic effect on the secretion of IL-1 beta and TNF-alpha when the reaction time was 18 or 24 hours. The secretion of cytokines was stimulated by low concentrations of the herbal extract, but inhibited by higher concentrations of the extract. Zingiberis rhizoma extract also showed a biphasic effect on the secretion of cytokine IL-1 beta when the reaction time was 18 or 24 hours. As for Cortex cinnamomi extract, no significant augmentative effect on cytokine secretion was found in our study. In consideration of the pyrogenic property of cytokines, it would appear that "hot nature" Chinese herbs can be further divided into different subgroups with minute differences based on their different effects on cytokine secretion.