Planta Med. 1996 Aug; 62(4): 293-6.

Inhibition of phospholipase C gamma 1 activity by amentoflavone isolated from Selaginella tamariscina.

Lee HS, Oh WK, Kim BY, Ahn SC, Kang DO, Shin DI, Kim J, Mheen TI, Ahn JS.

Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yusong, Taejon, Korea.

Amentoflavone was isolated as an inhibitor of phospholipase C gamma 1 (PLC gamma 1) and phosphoinositides (PI)-turnover in PLC gamma 1 overexpressing NIH3T3 fibroblasts (NIH3T3 gamma 1) from Selaginella tamariscina (Selaginellaceae) together with other related biflavonoids, isocryptomerin and cryptomerin B. Only amentoflavone inhibited the PLC gamma 1 activity with an IC50 of 29 microM and the formation of total inositol phosphates (IPt) in PDGF-stimulated NIH3T3 gamma 1 with an IC50 of 9.2 microM but did not show inhibitor activity against protein kinase C. Isocryptomerin and cryptomerin B did not show inhibitor activity against PLC gamma 1 at the concentration of 150 microM, and did not inhibit IPt production in PDGF-induced NIH3T3 gamma 1 at the concentration of 180 microM.