Liposarcoma: Comparison of flow cytometric and image cytophotometric DNA measurements

Author(s): Herget, G. W., Adler, C. P.

Journal/Book: Oncol Rep. 1996; 3: C/O D a Spandidos, 48 Vas Constantinou Ave, Athens 11635, Greece. Natl Hellenic Res Foundation. 559-562.

Abstract: Using nuclear suspensions, the DNA-content of 7 bone-associated, paraffin-embedded liposarcomas and 3 lipomas were examined by means of flow cytometry (FCM) and was compared with the results of image cytophotometric measurements (ICM). Using ICM, 6 of 7 liposarcomas showed an aneuploid DNA content (86%), whereas FCM showed aneuploidy in 3 cases (43%). None of the lipomas had an aneuploid DNA-content using either method. The correspondance of the two methods was 70%. Because aneuploidy is of prognostic importance, ICM could be the method to determine the DNA content of liposarcoma.

Note: Article CP Adler, Univ Freiburg, Inst Pathol, Referenzzentrum Knochentumoren, Ludwig Aschoff Haus, Albertstr 19, D-79002 Freiburg, Germany

Keyword(s): liposarcoma; image cytophotometry; flowcytometry; prognosis; PARAFFIN-EMBEDDED TISSUE; SOLID TUMORS; ANEUPLOIDY; SARCOMA; PLOIDY

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