Enhancement of human NK and LAK cytotoxicity against HCMV-infected cells by rhamnogalacturonan: specificity of reaction.

Journal/Book: Viral-Immunol 9 (1), 27-34. 1996;

Abstract: Human peripheral blood mononuclear cells (PBMC) and their subpopulationsobtained from healthy donors were used to study improvement ofMHC-unrestricted cytotoxic reactions against cells infected with humancytomegalovirus (HCMV) at different multiplicities of infection. Naturalkiller (NK) and lymphokine-activated killer (LAK) cytotoxicity againstHCMV-infected cells was greatly enhanced in the presence ofrhamnogalacturonan (500 ng/ml). The increase of the multiplicity ofinfection from MOI 0.1 to 1.0 had only a slight effect on cytotoxicityenhancement by rhamnogalacturonan. The chemical specificity ofinteraction of rhamnogalacturonan with effector cells and virus-infectedcells was found to be analogous to the interaction with tumor cells,i.e., both types of target cells must express a receptor forrhamnogalacturonan since rhamnogalacturonan-mediated enhancement of NKand LAK cytotoxicity against HCMV-infected cells was similarly inhibitedby preincubation of CD56+ effector cells with 60% deacetylated D-mannosepentaacetate. Author.

Keyword(s): CELLS-CULTURED

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