Differential endocytosis of mistletoe lectin I (ML I) and isolated subunits in murine L 1210 leukemia cells

Journal/Book: In:Lectins: Biol. Biochem. Clinical Biochem. van Driessche E. et al.(eds.) Vol. 8, pp. 48-54, Textop, Hellerup (Denmark). 1993;

Abstract: Mistletoe lectins and viscotoxins, which up to now have been isolatedonly from plant material, were detected and quantitatively determined inthe mistletoe preparation Iscador and in a fermented mistletoe extract.Lectins were isolated by affinity chromatography and analyzed byisoelectric focussing. Thus, in Iscador and in the fermented mistletoeextract only the mistletoe lectins ML II/III were found whereas typicalproteins of the ML I complex were missing. The lectin content of thepreparation and the extract was determined by "single radialimmunodiffusion" (SRID). For identification and quantitativedetermination of viscotoxins, a HPLC method was designed. Author.

Keyword(s): CHROMATOGRAPHY-AFFINITY

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