Zhongguo Zhong Yao Za Zhi. 1992 Jun; 17(6): 332-6, 381-2.
[Cytological observation on culture of embryo in vitro of Tripterygium hypoglaucum (level.) Hutch.]
Cytotoxicity Department of Kunming Medical College.
This paper for the first time reports the embryo culture in vitro of Tripterygium hypoglaucum. Callus, adventitious roots and adventitious protuberances were induced from the cultured embryos in vitro. Multi-buds at the excised embryo tip were also observed at the same time. Cytohistological examination showed (1) callus was histologically easy to be induced to form adventitious roots; (2) callus on the cotyledons was originated from the epidemical and subepidemical cells of the cotyledons, and the adventitious roots were originated from the disperse meristematic tissue or vascular tissue in the callus obtained from cultured excised embryos.