Analysis of tumor necrosis factor a- specific, lactose- specific and mistletoe lectin- specific binding sites in human lung carcinomas by labelled ligands

Journal/Book: Virchow Archiv A, Pathol. Anatomy Histopathol. 421 (4), 345-349. 1992;

Abstract: Receptor sites can be visualized by labelled ligands as an alternativeto receptor-specific antibodies, as substantiated for two differentreceptor classes. Recombinant tumour necrosis factor alpha (TNF) wasbiotinylated via amino-groups and the resultant probe was applied toformalin-fixed, paraffin-embedded tissue sections of 94 primarybronchial carcinomas and to normal peripheral lung parenchyma. Inaddition, monoclonal antibodies specific for neuron-specific enolase(NSE) and TNF itself were used. The biotinylatedbeta-galactoside-specific mistletoe lectin, which exhibitsdose-dependent immunomodulatory and toxic potency, and two probes thatspecifically detect certain types of sugar receptors were employed toillustrate further the feasibility of using ligands for receptorlocalisation. The tumours comprised 62 small cell lung carcinomas, 10epidermoid carcinomas, 11 adenocarcinomas and 11 large cell anaplasticcarcinomas. Expression of TNF-binding sites was found in 39 of the smallcell lung carcinomas and in 13 of the non-small cell lung carcinomas.Binding capacity for the TNF-specific antibody was seen in similarproportions of small cell lung carcinomas and of non-small cell lungcarcinomas. None of the normal lung parenchymas revealed significantstaining. Binding capacities to mistletoe lectin were seen in all normallung parenchymas and in nearly all cases of adenocarcinoma (10/11). Acorrelation between the expression of NSE and the binding capacities toTNF was detected. Endogenous lectins, specific for lactose orbeta-GalNAc, were displayed in nearly one half of the small cell lungcarcinoma cases (44% or 45% respectively) and in about 25% of thenon-small cell lung carcinoma cases. Author.

Keyword(s): ACETYLGALACTOSAMINE/ME (metabolism)

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