The activitiy of two immunotoxins composed of monoclonal antibody MoAb- 16 and A- chain of ricin (MoAb-16-RTA) or A- chain of mistletoe lectin I (MoAb-16-MLIA)

Journal/Book: Arch. Immunologiae et Therapiae Experimentalis 40, 223-227. 1992;

Abstract: The A-chains of ricin obtained from Ricinus communis or mistletoe lectinI from Viscum album were coupled to the monoclonal, anti-L1210V antibodyMoAb-16, using SPDP as a cross linking agent. The cytotoxic activity invitro of these immunotoxins was compared. Each of two immunotoxinstested, applied in vitro for 1 h in appropriate doses, causedirreversible inhibition of leukemic L1210 cells proliferation.Unexpectedly, MoAb-16-MLIA immunotoxin appeared to be cytotoxic tonormal bone marrow progenitor cells, as observed in NCFUS tests.Moreover, this immunotoxin revealed cytotoxic effect to the P388leukemia cells which do not share the antigen, common within L1210leukemia cells, detected by MoAb-16 antibody. Author.

Keyword(s): ANIMAL

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