Immunotoxin with mistletoe lectin I A-chain and ricin A-chain directed against CD5 antigen of human T-lymphocytes; comparison of efficiency and specificity

Journal/Book: Int-J-Immunopharm 13 (7), 1037-1041. 1991;

Abstract: Monoclonal anti-CD5 antibody was coupled to the enzymatically activesubunit of plant toxin (either mistletoe lectin I (ML) or ricin). Theobtained conjugates proved to be selectively toxic to CD5-bearing targetcells. The immunotoxin prepared from ML A-chain (MLA) was as toxic asnative ML and approximately 80-fold more active than the correspondingconjugate with ricin A-chain (RTA). The comparative studies of thestructural properties of isolated MLA and RTA were carried out usingintrinsic fluorescence spectroscopy. The results showed similarproperties for both proteins. No antigenic cross-reactivity against bothtoxins was detected when using polyclonal antibodies. The resultssuggest that MLA-antibody conjugates may be potential candidates fortherapeutical use.

Keyword(s): Medical:t-lymphocyte

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