Differentiation of rodent trypanosomes of the subgenus Herpetosoma bylectins.

Journal/Book: Trop-Med-Parasitol 1989 Sep, VOL: 40 (3), P: 273-8, ISSN: 0177-2392.. 1990;

Abstract: Trypomastigote forms of Trypanosoma microti, T. evotomys, T. grosi, T.musculi, and T. lewisi and trypomastigote and epimastigote forms of T.acomys were differentiated using 34 lectins and the Aminoff test forN-acetylneuraminic acid (NANA). Twelve of the lectins failed toagglutinate any of the above species. The number of lectins whichagglutinated each species differed; T. mciroti, T. evotomys, T. grosi,T. musculi, T. lewisi and T. acomys (trypomastigote and epimastigoteforms) were agglutinated by 7, 14, 7, 13, 11 and (11 and 10) lectinsrespectively. Some of the lectins were common in agglutinating allspecies of parasites, for example Bauhinia purpurea, Caragana aborescensand Viscum album. The minimum concentration of lectins whichagglutinated each parasite was quite different. The agglutinations werecell body-cell body, flagellum-flagellum or flagellum-cell body. Most ofthe agglutinations were inhibited by their specific carbohydrates. Thelowest concentrations of NANA was observed in T. lewisi (0.3micrograms/ml) and the highest in T. musculi (4.5 micrograms/ml). Theconcentrations of NANA in T. microti, T. grosi and in T. acomys(trypomastigote and epimastigote forms) were 2, 1.8, 2.9, and (1.4 and1.2) micrograms/ml respectively. Author.

Keyword(s): AGGLUTINATION

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