A method for demonstration of lectin-binding sites using a blood group substance A/ferritin conjugate.

Journal/Book: Acta-Histochem 84 (1), 77-82. 1989;

Abstract: A new technique for demonstration of lectin-binding sites is proposed.At the 1st step of the method, the lectin binds the respectivemonosaccharide constituent of the glycoconjugate and at the 2nd step,the blood-group substance A-ferritin conjugate is used as a marker. Thefollowing lectins were tested: wheat germ agglutinin, soybeanagglutinin, mistletoe lectin I, and concanavalin A. Human red bloodcells were used as material. By means of control experiments, thespecificity of the proposed technique was proved. Author.

Keyword(s): ABO-BLOOD-GROUP-SYSTEM

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