Detection and quantification of ligand leakage from lectin affinity columns

Journal/Book: Biomed. Biophys. Acta 48, 221-226. 1989;

Abstract: The usefulness of lectin affinity chromatography for the preparation ofglycoproteins is impaired by ligand release. Ligand leakage frommistletoe lectin (MLI) Sepharose 4B column was detected by 24 h skinreaction in mice and by immunoblotting. Immunoaffinity chromatographywas found to be an efficient method for the separation of lectin tracesfrom the glycoprotein fraction. A sugar concentration dependent increaseof lectin release from MLI-Sepharose 4B column was detected by a solidphase enzyme immunoassay. Author.

Keyword(s): ANIMAL

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