The site of action of the A- chain of mistletoe lectin I on eukaryotic ribosomes

Journal/Book: FEBS Letters 231 (2), 378- 380. 1988;

Abstract: The site of action of the A-chain of mistletoe lectin (ML-A) from Viscumalbum on eukaryotic ribosomes was studied. Treatment of rat liverribosomes with ML-A, followed by treatment of the isolated rRNA withaniline, caused the release of a fragment with about 450 nucleotidesfrom 28 S rRNA. Further analysis of nucleotide sequences of thisfragment revealed that the aniline-sensitive site of phosphodiester bondwas between positions A-4324 and G-4325 in 28 S rRNA. These resultsindicate that ML-A inactivates the ribosomes by cleaving a N-glycosidicbond at A-4324 of 28 S rRNA in the ribosomes as ricin A-chain does.Author.

Keyword(s): ANIMAL

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