Effects of lectin I from mistletoe (ML I) and its isolated A and B chains on human mononuclear cells: mitogenic activity and lymphokine release.

Journal/Book: Pharmazie 42 (5), 337-340. 1988;

Abstract: The interaction of lectin I from mistletoe (Viscum album) (ML I) and itsisolated A and B chains with mononuclear cells from healthy human donorswas investigated with respect to proliferation capacity (mitogenicity)and monokine/lymphokine factor (MSF) production. The factor produced wasstudied by means of the electrophoretic mobility inhibition assay usingguinea pig macrophages as indicator cells. ML I and its B chainexhibited comparable inhibitory effects on the proliferation ofmononuclear cells (MNC, lymphocytes) that differed in quantity only.After 72 h the tritiated thymidine incorporation had diminished incomparison to the control over a concentration range from 10(-7) to10(-11) mol/l (ML I) or from 3 X 10(-7) to 3 X 10(-9) mol/l (B chain),respectively. The ML I was about 30 times more active than the B chain.Moreover, MSF production could be substantiated for the B chain at aconcentration of 3 X 10(-8) mol/l. In contrast to the B chain, the Achain stimulated the MNC to blastogenic transformation at concentrationsof 3 X 10(-8) and 3 X 10(-9) mol/l. The stimulation index was much lowerthan after PHA stimulation. The same concentrations induced theproduction of lymphokine (MSF). The lymphokine activity on the indicatorcells could be inhibited by L-fucose. Perhaps both cytotoxicity andlymphocyte stimulation are important for anti-tumor activity afterapplication in vivo. Author.

Keyword(s): HUMAN

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