Identification of the normal microglial population in human and rodent nervous tissue using lectin-histochemistry

Journal/Book: Neuropathol-Appl-Neurobiol 14 (3), 221-227. 1988;

Abstract: Mistletoe lectin-1 (ML-1) and Ricinus communis agglutinin-120 (RCA-1)both possess D-galactose-specific surface-binding sites. They were usedto selectively identify microglial populations in aldehyde-fixed normalbrain tissue by lectin immunohistochemistry on paraffin and frozensections. Mistletoe lectin-1 was superior to RCA-1 in labellingmicroglia in the rat brain, whereas RCA-1 labelled human microgliabetter than ML-1. Thus, RCA-1 and ML-1 supplement each other foridentifying microglial in human and rodent central nervous systemtissues. The high reproducibility of the results and the applicabilityof the technique to routine histology, using formalin-fixed tissue,should facilitate study of the histogenesis and role of microglia in theCNS. Author.

Keyword(s): BRAIN/IM (immunology)

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