The toxic A-chain of mistletoe lectin I: Isolation and its effect on cell-free protein synthesis

Journal/Book: Acta biol. med. germ. 41 (4), K9-K16. 1982;

Abstract: The reduction of Sepharose bound mistletoe lectin I (ML I) is aconvenient method for preparing A- and B-chains under mild conditions.The B-chain represents the D-galactose binding moiety. The A-chain showsa significant dose-dependent inhibition of protein synthesis incell-free systems from rat liver and rabbit reticulocytes, while pureB-chain preparations do not affect ribosomal translation processes.Author.

Keyword(s): ANIMAL

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