Electron microscopic visualization by the unlabelled lectin/antiferritin-antibody/ferritin method of lectin receptors on cell surfaces

Journal/Book: Acta histochemica, Suppl.-Bd. XXII, 131-134. 1980;

Abstract: For electron microscopic demonstration of carbohydrate moieties on cellsurfaces of HeLa-cells the lectins from Viscum albumin, Canavaliaensiformis and Dolichos biflorus have been used. The stainingexperiments were performed by reaction of the cell surface receptorlocalized lectin with purified antiferritin-antibody followed byferritin. The three-step reaction cell surface receptors leads to lectinleads to antiferritin antibody leads to ferritin is proposed as generalmethod for electron microscopic localization of lectin receptors withoutcovalent coupling. Author.

Keyword(s): ANTIBODIES

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