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November 2022

Affinity chromatography of human serum proteins using matrix bound lectins from VISCUM ALBUM and VICIA FABA

Journal/Book: Acta biol. med. germ. 38, 697-700. 1979;

Abstract: The D-galactose specific lectin from Viscum album and the D-mannosespecific lectin from Vicia faba react with serum proteins which containthe corresponding carboyhydrate moieties. By affinity chromatography ofhuman serum using the combination of insolubilized lectins coupled toSepharose it is possible to fractionate serum proteins in 3 groups: 1.proteins which react with both of the lectins; 2. proteins which reactonly with one of the lectins; 3. proteins which do not display anyaffinity for one of the two lectins. Author.

Keyword(s): BLOOD-PROTEINS/ IP (isolation & purification)

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