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October 2021

Trypsin-Induced Phospholipase Activity in Human Platelets

Author(s): Jesse, R., Cohen, P.

Journal/Book: Biochem J. 1976; 160: 405-408.

Abstract: The technique of crossed immunoelectrophoresis was used to analyse the effects of bromelain on the antigens contained within the membranes of mouse erythrocytes. Immune sera were obtained from pats goats had been injected with membranes prepared from either normal erythrocytes or bromelain-modified erythrocytes. Precipitation patterns obtained by crossed immunoelectrophoresis showed that treatment of erythrocytes with bromelain changed the electrophoretic mobility of a major protein antigen and increased the solubility of two membrane gIycoproteins. The possibility that enzymic modifcatian of the erythrocytes led to the creation of entirely new antigenic determinants was not supported by the precipitin patterns obtained using sera adsorbed with various membrane preparations.

Keyword(s): Erythrocyte membrane

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