A Colorimetric Method for the Determination of Mucopolysaccharides and Other Acidic Polymers1

Journal/Book: Reprinted from ANALYTICAL BIOCHEMISTRY Volume 29 Nr. 3 June 1969 S.421-432. 1969;

Abstract: With the technical assistance of Janice L. Yager Department of Biochemistry College of Medical Sciences University of Minnesota Minneapolis Minnesota 55455 SUMMARY A colorimetric method for the estimation of small amounts (0.5 to 5 µg) of acidic polysaccharides has been developed using the shift in the visible absorption spectrum of a carbocyanine dye when it is bound to a polyanion. The limitations of the method including the evaluation of interfering substances have been investigated. The method has the advantages of simplicity and rapidity lacking in methods presently used for mucopolysaccharides and other acidic polysaccharides. The absorbance in the region between 600 and 650 mµ was found to be a linear function of the polyanion concentration. . . .

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