Chromatographic Separation of Vitamins D2 and D3 and Related Compounds1

Abstract: Department of Biochemistry University of California Riverside California 92502 SUMMARY A reversed-phase chromatographic system is described that is capable of separating vitamin D2 from vitamin D3 and ergosterol from 7-dehydrocholesterol and cholesterol. The method uses Factice a polymerized and vulcanized soybean oil as stationary phase and a water/acetone mitxure as mobile phase. In contrast to other previously published techniques capable of separating vitamins D2 and D3 the present separation is accomplished without any chemical modification or alteration of the D-vitamins. . . .

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